concentration sodium hypochlorite effects on allium cepa

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Genotoxicity of the disinfection byproducts resulting ...

To evaluate the effect of FeSO 4, the results obtained with each disinfectant with and without FeSo 4 were compared by Students's ttest. Allium cepa anaphase aberration test. For the Allium root anaphase aberration assay (Grant, 1982; Fiskesjo, 1985) equalsized young bulbs of common A. cepa (2– cm diameter) were used. Bulbs were exposed ...

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Germination and antioxidant defense system in onion ...

Germination and antioxidant defense system in onion (Allium cepa . ... Onion ( Allium cepa L.) belonging to the family of Amaryllidaceae, is the second most important horticultural crop ... sterilized by using 70 % alcohol and then sterilized with 2 % sodium hypochlorite, then thoroughly washed several times with distilled water. The experiment ...

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Plant Tissue Localization and Morphological Conversion of ...

Interaction with Allium cepa L. Leidong Hong 1,2, ... confer beneficial effects on the host plants, including the enhancement of growth and productivity, and in return, PGPB benefit from the nutrients ... ethanol for 1 min, 2% (v/v) of sodium hypochlorite with % (v/v) of Tween 20 for 10 min, and then

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International Journal of Current Research in

MarinMorales, 2009). The Allium cepa test, which is considered one of the efficient approaches to assess toxic effects of environmental chemical, was selected to aveluate the potencial risks of these ubiquitous pollutants (Herrero et al., 2012). Biomonitors, like Allium cepa L., wich is one of the

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EFFECTS OF SODIUM HYPOCHLORITE ON SOME …

Keywords: Sodium hypochlorite, Physiological parameters, Chromosomal aberration Abstract The effects of sodium hypochlorite (NaClO) on the seed germination, seedling growth (radicle length, radicle number and fresh weight), mitotic activity and chromosomal aberrations of Allium cepa L. germinated under salt stress were studied.

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EFFECT OF SALT STRESS (NaCl) ON MORPHOLOGICAL …

(Allium cepa L.) SEEDLINGS ... In order to study the effects of salt stress, using sodium chloride. In the present study seeds of four onion cultivars ... then sterilized with 2 % sodium hypochlorite, then thoroughly washed several times with distilled water. The experiment was carried out using five salinity levels (0, 50, 100, 150 and 200 mM ...

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Efficacy of chlorine, peroxyacetic acid and mildheat ...

Calçots are the immature floral stems of the secondyear onion (Allium cepa L.) resprouts with economic importance in Spain. The effect of sodium hypochlorite, peroxyacetic acid and mild heat treatment on the microbiological, physicochemical and visual quality of calçots after disinfection and the storage period of 15 d at 4 ºC was studied.

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Ecological Effects Test Guidelines OPPTS Seed ...

(4) If the rangefinding test showed that the highest concentration of the chemical tested (not less than 1,000 mg/L) had no effect on the test species, report the results by species and concentration and a statement that the chemical is of minimum phytotoxic concern, (5) If the rangefinding test showed greater than 50 percent inhibition of ...

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EFFECT OF SALT STRESS (NaCl) ON MORPHOLOGICAL …

EFFECT OF SALT STRESS (NaCl) ON MORPHOLOGICAL PARAMETERS OF ONION (Allium cepa L.) SEEDLINGS ... Selected seeds were surface sterilized with 2 % sodium hypochlorite…

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A scanning electron microscope evaluation of efficacy of ...

Context: Sodium hypochlorite (NaOCl) is the most widely used endodontic it fulfills most of the requisites of an ideal irrigant, it has certain drawbacks, chiefly severe softtissue damage. Allium sativum (garlic extract) is a proven antimicrobial agent that has the ability to dissolve inorganic tissue with minimal toxicity. However, its ability to remove smear layer (SL) is ...

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Alleviation of Adverse Effects of Copper on Allium cepa L ...

Alleviation of Adverse Effects of Copper on Allium cepa L. …. 3 subsequently electrical conductivity of the solution (EC1) was were then autoclaved at 120 C for 20 min and the final electrical conductivity (EC2) was obtained after equilibration at 25 C. Leaf cell MSI was determined according to the following formula

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IDENTIFICATION AND IN VITRO CONTROL OF THE CAUSAL …

disinfected with 2% sodium hypochlorite for 2 min, then washed three times with sterile distilled water and placed in PDA culture medium. The morphological characteristics of the colonies and reisolated fungi were compared with the original strains to verify Koch's postulates and the whole procedure was performed twice.

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THE EFFECT OF COLCHICINE ON ROOT MITOSES IN ALLIUM

and RUTTLE (1938), who first studied the effect of colchicine on plant cells (stamen hairs of Tradescantia), arrived at a similar result. The material of the experiments dealt with in the present paper consists of root tips of Allium fistulosum and Cepa. Root tips of A. fistulosum were secured from bulbils that had overwintered in the

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Cytotoxic evaluation of sodium hypochlorite, using Pisum ...

May 30, 2019· The objective of this study was to evaluate the cytotoxic effect of different sodium hypochlorite concentrations, using apical root cells of P. sativum as a bioindicator. Initially, the seeds of P. sativum were exposed to different concentrations of sodium hypochlorite (, , , , , 2 mg L −1) and to a control solution based on distilled water.

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Bacteria in onions during the growing season. ASSELIN, ...

Effect of variety and production environment on the chemical composition of western long ... Allium species such as onion ... Grower trials of sodium/potassium hypochlorite sprays on growing onions to reduce bacterial rot in New York onions. BONASERA, J., Asselin and S. V. Beer. Plant Pathology and PlantMicrobe Biology, ...

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Science and Education Publishing Open Access Peer ...

The study was undertaken to develop an efficient protocol for in vitro regeneration of shallot ( Allium cepa ). Two local shallot varieties (Huruta and Minjar) were used as experimental materials where basal discs were used as explants. Murashige and Skoog medium supplemented with different concentrations and combinations of 2, 4Dichlorophenoxyacetic Acid, 6Benzylaminopurine, Kinetin and α ...

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Allium cepa L. and Quercetin Inhibit RANKL/ gingivalis LPS ...

Allium cepa L. and Quercetin Inhibit RANKL/Porphyromonas ... with 500mL of % sodium hypochlorite for 5min. The plates were then aspirated, washed thoroughly with water, ... optimal concentration of AcE (1000𝜇g/mL) or Qt (5𝜇M)

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The Effects of Trichoderma harzianum on Germination of ...

In the T. harzianumfree According to results, it is clear that there is a possitive treatment, increased salinity levels resulted in decreased effect of T. harzianum on during germination stages of germination, inhibition of seedling growth and increased onion (Allium cepa L.) seeds under salt stress conditions.

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IN VITRO MICROPROPAGATION IN TROPICAL SHORT DAY …

DAY ONION (ALLIUM CEPA L.) RUPALI PASSI, AS DHATT AND MK SIDHU* ... the present study was planned to study the effect of varieties ... sodium hypochlorite % and sodium hydroxide %) as per the durations mentioned in Table 1. The explants were washed with sterile water for

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Cytotoxicity, Genotoxicity, and Phytotoxicity of Tannery ...

The effect of collected TE on Allium cepa showed a significant reduction in root length, decrease in dry weight, increase in root diameter, and increase in root hairs. The estimated EC 50 value of Allium cepa exposed to TE was found to be % which is almost similar to the findings of Gupta et al. (2011) .

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Heat Treatments Control Extension Growth and Enhance ...

Allium cepa x Allium fistulosum, leaf base, leaf extension, hot water dips, sodium hypochlorite Abstract. Extension growth of minimally processed (removal of roots and compressed stem) green onions (Allium cepa L. x A. fistulosum L.) was greatly reduced by storage in ... determine the effect …

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Cytotoxic and genotoxic effects of sodium hypochlorite on ...

Chlorination is widely used method in the disinfection of drinking and utility water worldwide. In this study, cytotoxic and genotoxic effects of sodium hypochlorite were investigated by the cytokinesisblock micronucleus assay and chromosomal aberration analysis on human peripheral lymphocytes in vitro.

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1. Background Hindawi Publishing Corporation

The effect of collected TE on Allium cepa showed a significant reduction in root length, decrease in dry weight, increase in root diameter, and increase in root hairs. The estimated EC 50 value of Allium cepa exposed to TE was found to be % which is almost similar to the findings of Gupta et al. (2011) [40].

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Effect of salt stress (NaCl) on morphological parameters ...

Salinity is one of the major environmental factor, which affects on plant growth and development of crops worldwide, the present research is performed with four onion cultivars. Which include Agrifound dark red (AFDR), Agrifound white (AFW), LINE28 and Krishnapuram (KP) were collected from NHRDF (National Horticulture Research and Development Foundation) Kurnool and from farmers.

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Influence of Temperature on Mutagenicity in Plants Exposed ...

Allium cepa. Test . After root germination under controlled laboratory con ditions (20˚C in mineral water) young bulbs of . Allium cepa. of equal size (2 cm in diameter) were exposed to disinfected and raw water for 24 and 72 hours at 10˚C, 20˚C and 30C. At the end of exposure roots were cut ˚

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Study the effect of the type of packaging and treatment ...

Study the effect of the type of packaging and treatment sodium hypochloride and licorice extract in percentages of weight loss and total damage in class onion taxas early grano. ... Allium cepa allium cepa Subject Category: Organism Names see more details, ... sodium hypochlorite. storage quality. temperature. Show more.

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fn sivo Biological Evaluation of Crude Extracts of ...

fn sitro and fn sivo Biological Evaluation of Crude Extracts of aioscorea ... healthy onion bulbs were properly surface sterilized using 99% ethanol and sodium hypochlorite (bleach). The sterile onion bulbs were then sprayed with the extracts at different concentrations two ... The onion (Allium cepa L.), also known as bulb (Fritsch and Friesen ...

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Cytotoxic and genotoxic effects of sodium hypochlorite on ...

May 30, 2009· Chlorination is widely used method in the disinfection of drinking and utility water worldwide. In this study, cytotoxic and genotoxic effects of sodium hypochlorite were investigated by the cytokinesisblock micronucleus assay and chromosomal aberration analysis on human peripheral lymphocytes in vitro.

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